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KMID : 1024420100140030263
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Food Engineering Progress
2010 Volume.14 No. 3 p.263 ~ p.270
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Isolation and Identification of Alkalophilic Microorganism Producing Xylanase
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Choi Ji-Hwi
Bai Dong-Hoon
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Abstract
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An alkalophilic microorganism named DK-2386, which produces xylanase, was isolated from soil of Taejo-mountain, Cheonan-si, Chungnam, Korea. The isolated strain was characterized as Gram-positive, with size of 0.4×2.5 μm, spore forming, anaerobic, catalase positive, possessed with hydrolysis abilities of casein, starch, sodium carboxy methyl cellulose, and xylan, reduction of nitrate to nitrite, resistant against lysozyme, urease positive, and motility positive. The color of culture broth was reddish yellow. The strain DK-2386 was identified as Bacillus agaradhaerens by whole cell fatty-acid composition analysis and 16S rDNA sequence analysis. However, it was not identical to Bacillus agaradhaerens 40952 obtained from the Korean Culture Center of Microorganism in its colour of culture broth. Therefore, we have named the newly isolated strain as Bacillus agaradhaerens DK-2386.
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KEYWORD
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Bacillus agaradhaerens, alkalophiles, xylanase
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